Table 3.
Fixed-effects meta-analysis of top candidate SNPs for difference test interactions between SSB consumption and SNPs on HDL-C and TG concentrations in CHARGE consortium cohorts and UKB*
| SNP | Location (Hg19) | Alleles (E/A)† | MAF | SSB Intake Category | n | Effect Size (SE) | P-value | Direction‡ | I2 | p Diff |
|---|---|---|---|---|---|---|---|---|---|---|
| HDL-C (mg/dl) | ||||||||||
|
| ||||||||||
| rs71556729§ | 72989516 | T/C | 0.05 | Low | 68,701 | 0.01 (0.05) | 0.81 | ++ | 0 % | 1.5E-06‖ |
| High | 15,227 | 2.06 (0.44) | 3.48E-06 | ++ | 74 % | |||||
|
| ||||||||||
| rs35709627§ | 72999171 | A/G | 0.05 | Low | 69,667 | 0.01 (0.04) | 0.74 | ++ | 0 % | 1.0E-05‖ |
| High | 15,883 | 1.37 (0.32) | 2.15E-05 | ++ | 87 % | |||||
|
| ||||||||||
| rs71556736 | 73034929 | T/C | 0.13 | Low | 69.667 | 0.02 (0.02) | 0.33 | ++ | 93 % | 2.5E-05‖ |
| High | 15,882 | 0.84 (0.20) | 3.27E-05 | ++ | 42 % | |||||
|
| ||||||||||
| rs73137017 | 72974413 | G/A | 0.04 | Low | 69,303 | 0.01 (0.05) | 0.82 | ++ | 0 % | 0.04 |
| High | 15,783 | 0.73 (0.37) | 0.05 | ++ | 81 % | |||||
|
| ||||||||||
| rs79578725 | 73002455 | A/G | 0.05 | Low | 68,929 | −0.02 (0.04) | 0.64 | −− | 21 % | 0.55 |
| High | 15,783 | −0.22 (0.36) | 0.53 | −− | 0 % | |||||
|
| ||||||||||
| TG (ln-mg/dl) | ||||||||||
|
| ||||||||||
| rs55673514 | 73021456 | G/A | 0.04 | Low | 69,096 | −0.002 (0.01) | 0.84 | +− | 29 % | 0.0005‖ |
| High | 15,395 | −0.06 (0.02) | 0.001 | −− | 0 % | |||||
|
| ||||||||||
| rs799157 | 73020301 | T/C | 0.05 | Low | 70,235 | 0.03 (0.01) | 2.55E-07 | ++ | 59 % | 0.05 |
| High | 16,006 | 0.06 (0.02) | 0.0002 | ++ | 19 % | |||||
Top candidates represent statistically significant or suggestive interactions (pDiff<0.005 or pinteract<0.005) in CHARGE cohort meta-analysis. Models adjusted for age, sex, total energy intake, field center and accounted for family or population structure where applicable plus education, smoking, physical activity, alcohol intake, and body mass index (kg/m2). For the difference test, interaction coefficients are shown as β (SE), where β represents the direction and magnitude of change in the outcome trait with each additional effect allele among participants with low (CHARGE:<1 serving/month; UKB: non-consumers) or high (CHARGE: >1 serving/day; UKB: consumers) SSB consumption.
Alleles presented as effect (E)/alternative (A) alleles
Order of cohorts for regression coefficient directions: CHARGE cohorts, UKB (+, positive effect size; −, negative effect size).
Linkage disequilibrium (R2) between rs13240662 and rs71556729=0.55 in European ancestry groups of Phase 3 (Version 5) of the 1000 Genomes Project.
Indicates a statistically significant interaction based on Bonferroni-corrected pDiff<0.01 (0.05/5 top signals) for HDL-C and pDiff<0.025 (0.05/2 top signals) for TG concentrations
CHARGE, Cohorts for Heart and Aging Research in Genetic Epidemiology; HDL-C, high-density lipoprotein cholesterol; MAF, minor allele frequency; SNP, single nucleotide polymorphism; SSB, sugar-sweetened beverages; TG, triglyceride; and UKB, UK Biobank.