Table 2. SARS-CoV-2 isolates from BEI resources carrying mutations near the S1/S2 cleavage site.
All SARS-CoV-2 isolates available as of July 6th, 2021 from BEI resources that contain mutations near the S1/S2 cleavage site. Data was compiled using the certificates of analysis provided by BEI resources. Also included is a recombinant infectious clone derived from USA-WA1/2020 isolate found to contain two mutations in the S1/S2 cleavage site. Yellow cells indicate substitutions and red cells indicate deletions. Not included are the 17 other available isolates containing either no mutations at S1/S2 or containing mutations canonical to their lineage (e.g. P681R in B.1.617 or P681H in B.1.17). Mutations noted are relative to MN908947.3, the Wuhan-Hu-1 SARS-CoV-2 isolate.
| BEI Number | Name (SARS-CoV-2 isolate) | Lineage | Native Furin Site AA Seq | Frequency |
|---|---|---|---|---|
| 675- QTQTNSPRRAR^S −686 | ||||
| NR-53945 | Isolate hCoV-19/Scotland/CVR2224/2020 | B.1.222 | Mut: R682L | 0.060 |
| NR-52368 | Isolate New York 1-PV08001/2020 | B.4 | Mut: R682W | 0.402 |
| NR-52281 | Isolate USA-WA1/2020 | A | Mut: R682L | 0.159 |
| NR-52282 | Isolate Hong Kong/VM20001061/2020 | A | Mut: R682Q | 0.958 |
| NR-52386 | Isolate USA-CA4/2020 | B | Mut: R682L | 0.817 |
| NR-52387 | Isolate USA-CA2/2020 | B.2 | Mut: R682L | 0.587 |
| NR-52439 | Isolate Chile/Santiago_op4d1/2020 | A.2 | Mut: R685H | 0.989 |
| NR-53514 | Isolate New York-PV08410/2020 | B.1 | Mut: R682W | 0.439 |
| NR-53516 | Isolate New York-PV09158/2020 | B.1.3 | Mut: R682Q | 0.583 |
| NR-53565 | Isolate Canada/ON/VIDO-01/2020 | B | Mut: R685S | 0.463 |
| NR-54009 | Isolate hCoV-19/South Africa/KRISPK005325/2020 | B.1.351 | Mut: R682W | 0.902 |
| Mut: Q677H | 0.899 | |||
| NR-52284 | SARS-CoV-2, Isolate Italy-INMI1 | Not Stated | Mut: R682W | 0.209 |
| 679–686 del | 0.611 | |||
| NR-52359 | SARS-CoV-2, Isolate England/02/2020 | A | 679–686 del | 0.824 |
| NR-52370 | SARS-CoV-2, Isolate Germany/BavPat1/2020 | B | 679–684 del | 0.565 |
| NR-52381 | SARS-CoV-2, Isolate USA-IL1/2020 | B | 675–679 del | 0.613 |
| NR-52382 | SARS-CoV-2, Isolate USA-CA1/2020 | A | 675–679 del | 0.613 |
| NR-52383 | SARS-CoV-2, Isolate USA-AZ1/2020 | A | 681–687 del | 0.861 |
| NR-52384 | SARS-CoV-2, Isolate USA-WI1/2020 | B | 687–689 del | 0.527 |
| BEI Number | Name | Lineage | Result | Frequency |
| NR-54003 | SARS-CoV-2, Isolate USA-WA1/2020, Recombinant Infectious Clone with Nanoluciferase Gene (icSARS-CoV-2-nLuc) | A | A684E | 0.287 |
| A | S686G | 0.104 |