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. Author manuscript; available in PMC: 2022 Jan 18.
Published in final edited form as: Mucosal Immunol. 2021 Aug 21;15(1):74–83. doi: 10.1038/s41385-021-00441-3

Figure 5. PTPN2 deficiency in myeloid cells results in loss of macrophage response to IL-4 in vivo.

Figure 5.

WT and Ptpn2-LysMCre mice were injected intraperitoneally with IL-4-IL-4R immune complexes and A) peritoneal lavages analyzed for IL-10 and TGFβ after 24 h, B) peritoneal immune cells collected after 24 h and analyzed for mRNA expression of Chil3 and Arg1 normalized to WT controls and Gapdh; C) peritoneal immune cells collected after 2 h and STAT6 phosphorylation analyzed in peritoneal macrophages (gated as live, single, CD45+, CD11b+, Gr1-, CD64+ cells), and D) peritoneal immune cells collected 24 h after injection and macrophages analyzed for the proportion of CD206+ cells. Gated as in (B). * = p < 0.05, ** = p < 0.01, *** = p < 0.001, ANOVA with Holm-Sidak’s multiple comparisons test. Representative results from 2 independent experiments with 3–4 mice per group.