Skip to main content
. Author manuscript; available in PMC: 2022 Apr 1.
Published in final edited form as: Mol Cancer Ther. 2021 Aug 10;20(10):1904–1915. doi: 10.1158/1535-7163.MCT-20-0638

Figure 2.

Figure 2.

Itraconazole suppresses AKT phosphorylation in esophageal cancer cells. A, Phospho-kinase analysis of OE33 cells treated with 2.5 μM itraconazole or DMSO for 48 hours. The pixel density of marked phosphorylated AKT (left) relative to internal control is shown graphically (right). B, Upregulation of AKT activity in esophageal cancer. Western blot analysis of phosphorylated and total AKT expression in immortalized human esophageal squamous (NES-G2T, NES-B10T), Barrett’s esophagus (BAR-T and BAR-10T), ESCC (KYSE70 and KYSE510), and EAC (FLO-1 and OE33) cell lines. GAPDH is used as a loading control.