Fig. 2.

Complexome profile of mitochondrial matrix chaperones in ClpP^−/− brain. Digitoninsolubilized mitochondrial complexes were separated by high-resolution clear native electrophoresis (hrCNE) into 60 fractions and identified/quantified by mass spectrometry. Protein complexes in Coomassie-stained gels were further processed to present abundance profiles as a heatmap (with red > yellow color representing the highest abundance) and 2D plot (with each protein represented by differently colored line as coded in gel picture above). The absence of ClpP is clearly detected, and triggers not only marked accumulation of several downstream factors in the UPR^mt pathway, but also abnormal migration at unexpected high molecular sizes, particularly for the strongly accumulated co-chaperone DNAJA3