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. Author manuscript; available in PMC: 2021 Sep 9.
Published in final edited form as: Neurogenetics. 2021 Aug 3;22(4):297–312. doi: 10.1007/s10048-021-00657-2

Fig. 4.

Fig. 4

Cell fractionation in MEFs, comparing mitochondrial with nuclear (a) and cytosolic (b) compartments. Immunoblotting was used to control the purity of the fractionation with the mitochondrial marker PORIN, the nuclear marker LAMIN A/C, and the cytosolic marker GAPDH, comparing littermate WT and ClpP−/− cells of matched sex. The detection of DNAJA3 revealed ClpP deficiency to cause accumulation of DNAJA3 short isoform (S) in the mitochondria and nucleus, while STAT1 (87 kDa) accumulation occurred in the mitochondria and cytosol