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. Author manuscript; available in PMC: 2022 Oct 14.
Published in final edited form as: FASEB J. 2021 Nov;35(11):e21957. doi: 10.1096/fj.202100691R

Figure 4. Osteoblast/osteocyte-derived sclerostin regulates Pdgrfa+ adipoprogenitor cell behavior.

Figure 4.

(A) PCR analysis of Sost gene recombination in tissues of of male, 12week old osteoblast-specific Sost knockout (ObΔSost) mice. (B) Serum sclerostin levels in male, 12 week old control and osteoblast/osteocyte-specific Sost knockout (ObΔSost) mice (n=6–8mice/genotype). (C and D) Representative microCT images and quantification of bone volume per tissue volume in the distal femur of control and ObΔSost mice (n=8–9mice/genotype). (E) Body weight of control and ObΔSost mice fed a chow or high fat diet (HFD, 8 weeks on diet) (n=9–13mice/genotype). (F) Gonadal (gWAT) and inguinal (iWAT) fat pad weight (n=9–13mice/genotype). (G) Representative iWAT histological sections. 10X magnification. (H and I) Random fed blood glucose and serum insulin levels (n=6–9mice/genotype). (J and K) Glucose tolerance (GTT) and area under the curve (AUC) analysis (n=8–10mice/genotype). (L and M) Quantification of CD45−: Sca1+: PDGFRα+ adipoprogenitors in iWAT of chow-fed (L) and HFD-fed (M) mice (n=9–13mice/genotype). (N) PCR analysis of Sost gene recombination in the iWAT of male, 12week old control (Con) and adipocyte-specific Sost knockout (AdΔSost) mice. (O) Serum sclerostin levels (n=7–8mice/genotype). (P) Body weight (n=7–8mice/genotype). (Q) Gonadal and inguinal fat pad weight (n=7–8mice/genotype). (R) Quantification of CD45−: Sca1+: PDGFRα+ adipoprogenitors in iWAT (n=7–8mice/genotype). All data are represented as mean ± SEM. *p < 0.05.