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. Author manuscript; available in PMC: 2022 Jan 6.
Published in final edited form as: Clin Transl Oncol. 2021 Aug 9;24(1):127–144. doi: 10.1007/s12094-021-02677-8

Fig. 4.

Fig. 4

Chemosensitivity profiles of TU-BcX-4IC cells. A TU-BcX-4IC-derived cells and MDA-MB-231 cells were treated with the NCI-approved oncology drug panel for three days. Overall, TU-BcX-4IC cells were more chemoresistant than MDA-MB-231 cells. B TU-BcX-4IC adherent cells were resistant to paclitaxel treatment (10 nM, 72 h), observed with a live/dead Calcein-AM/EthD III fluorescent stain. Green = live cells, Red = dead cells. C U-BcX-4IC organoids were treated with varying doses of paclitaxel (1 μM, 10 μM, 100 μM) and stained with the live/dead cytotoxicity stain and Hoechst nuclear stain using the Pu·MA system (Protein Fluidics, Burlingame CA) and images were obtained using confocal microscopy (Molecular Devices, San Jose CA)