Table 2.

Specific genes involved in ECM organization, positive regulation of cell migration, and angiogenesis identified from RNA sequencing that were retained in both lower passage (T1) and higher passage (T4) tumors, compared to the original tumor (T0)

ECM Organization	Positive Regulation of Cell Migration	Angiogenesis
ADAMTSL4, ATP7A, CD44 CD47, SMOC2, TNFRSFllB, APP, B4GALT1, BGN, CCDC80, DCN, DAG1, ELN, FENl , FEN2, HSPG2, JAM2, KDR, LUM, LOXLl , NFl, NIDI , NID2, QLFML2B, POSTN, PXDN, PRDX4, SPPl , SPARC, SPINTl , SERPINBS, SERPINEl, THBSl, TGFBI, VCAMl, VCAN, VWF	APC, CCR, CXCL16, DAB2, HRAS, TNFAIP6, ACTN4, AIFl, BMP4, CREB3, CIBl , CARMILl, CTSH, CEMIP, F3, CSFIR, COROlA, DIAPHl , FERMT3, FLTl , GPNMB, HSPAS, HAS2, IGFlR, INSR, KDR, LGR6, MMP14, MCAM, MYADM, MYOlC, MYLK, NOTCHl , PIK3CD, PLAU, PDGFC, PDGFRB, PODXL, RACK I , RRAS2 , SERPINB3, SPAG9, SDCBP, THBSl , TGFBRl , VEGFA	ATPSB, CCL2, CXCL8, EPHB2, HTATIP2, NOXS, NUSl, ARHGAP22, SHCl , TNFRSF12A, THYl , ACVRLl , ANPEP, ANGPTL4, ANXA2, CIBl , CLIC4, CSPG4, DLL4, ERAPl , EPASl , EFNAl , ECMl , FLTl , FMNL3, FZD8, HMOXl , HSPG2, HEYl , HIFlA, KDR, MCAM, MFGE8, NOV, NRXN3, NRCAM, NRPl , TNC, NCL, PNPLA6, PTEN, PIK3CG, PLXNDl , SCG2, SERPINEl , SLC12A6, SY, TGFBl , TIEl , UNCSB, VEGFA VEGFC VASHl
COLlAl , COL1A2, COL3Al , COL4Al , COL4A2, COL4AS, COL4A6, COL9A3, COLSAl , COLSA2, COLSA3, COL6Al , COL6A2, COL6A3, COL8Al , COL16Al COL18Al COL27Al	COLlAl , COL18Al	COL4A2, COL8Al , COL8A2, COLlSAl , COL18Al ,
ITGAll , ITGA2, ITGA4, ITGAS, ITGAV, ITGAX, ITGBl , ITGB2, ITGAV ITGB3, ITGB4, ITGBS, ITGB6, ITGB8	ITGAS, ITGA6, ITGAV	ITGAS, ITGAV
LAMA3, LAMA4, LAMAS, LAMB1, LAMC1, LAMC2N	LAMB1, LAMC2	LAMAS
		MMP14, MMP2
	SEMA3C, SEMA3D, SEMA4A, SEMA4B, SEMA4D, SEMASB, SEMA6C	SEMA4A
ADAMTSL4	ADAMlO, ADAM17