Table 2. Summary of TCR-β and IgH repertoire analysis from term and preterm pregnancies grouped by entropy, CDR3 length, and SHM.
Comparisons between groups were assessed using the Wilcoxon rank-sum test.
| Term Maternal Blood |
PPROM Maternal Blood |
Term Cord Blood |
PPROM Cord Blood |
Term Maternal Blood vs. Term Cord Blood |
PPROM Maternal Blood vs. PPROM Cord Blood |
Term Maternal Blood vs. PPROM Maternal Blood |
Term Cord Blood vs. PPROM Cord Blood |
||
|---|---|---|---|---|---|---|---|---|---|
| Mean (SD) | Mean (SD) | Mean (SD) | Mean (SD) | p-value | p-value | p-value | p-value | ||
| TCR | Entropy (bit) | 12.87 (1.64) | 13.87 (0.93) | 13.53 (0.79) | 14.89 (0.99) | 3.52E-01 | 2.32E-02 | 1.54E-01 | 3.84E-03 |
| CDR3 Length (nt) | 36.60 (0.53) | 36.44 (0.38) | 35.91 (0.30) | 35.37 (0.76) | 1.35E-05 | 1.30E-04 | 7.09E-01 | 2.20E-02 | |
| IgD | SHM (rate) | 1.16E-2 (6.38E-3) | 9.63E-3 (2.78E-3) | 6.69E-3 (3.9E-4) | 6.65E-3 (3.0E-4) | 2.53E-05 | 7.58E-05 | 7.52E-01 | 7.52E-01 |
| IgD Unmutated | Entropy (bit) | 10.88 (1.88) | 12.20 (1.35) | 12.18 (1.02) | 12.90 (0.88) | 3.95E-02 | 1.65E-01 | 9.56E-02 | 5.59E-02 |
| CDR3 Length (nt) | 46.06 (0.92) | 45.91 (0.74) | 42.81 (1.02) | 40.31 (0.90) | 9.47E-07 | 1.08E-05 | 7.96E-01 | 1.02E-06 | |
| IgD Mutated | Entropy (bit) | 9.54 (2.19) | 10.66 (1.15) | 11.82 (0.36) | 11.98 (0.22) | 3.38E-04 | 1.05E-03 | 1.72E-01 | 1.92E-01 |
| CDR3 Length (nt) | 45.07 (1.75) | 45.01 (1.44) | 43.49 (0.99) | 41.10 (1.09) | 1.22E-02 | 1.08E-05 | 7.96E-01 | 7.14E-06 | |
| IgM | SHM (rate) | 1.76E-2 (2.23E-3) | 1.77E-2 (2.36E-3) | 1.16E-2 (5.7E-4) | 0.0117 (6.4E-4) | 4.95E-08 | 4.11E-05 | 8.77E-01 | 8.77E-01 |
| IgM Unmutated | Entropy (bit) | 11.20 (1.46) | 12.27 (0.98) | 12.41 (0.55) | 12.88 (0.4) | 2.41E-02 | 1.13E-01 | 4.56E-02 | 1.58E-02 |
| CDR3 Length (nt) | 44.67 (0.75) | 44.82 (0.76) | 41.70 (0.90) | 39.70 (0.85) | 9.97E-08 | 4.11E-05 | 5.16E-01 | 7.34E-05 | |
| IgM Mutated | Entropy (bit) | 10.26 (1.35) | 10.76 (0.99) | 12.33 (0.19) | 12.44 (0.16) | 3.34E-06 | 1.65E-04 | 5.57E-01 | 6.22E-02 |
| CDR3 Length (nt) | 41.97 (1.19) | 42.43 (1.69) | 42.27 (0.92) | 40.43 (1.06) | 8.04E-01 | 5.63E-03 | 6.88E-01 | 4.70E-04 | |
| IgA | Entropy (bit) | 7.78 (1.12) | 8.00 (0.81) | 2.89 (2.54) | 3.59 (1.76) | 2.88E-04 | 4.11E-05 | 6.66E-01 | 6.66E-01 |
| SHM (rate) | 8.36E-2 (1.14E-2) | 8.07E-2 (7.9E-3) | 1.68E-2 (2.8E-2) | 5.21E-3 (9.08E-3) | 4.94E-04 | 4.11E-05 | 2.97E-01 | 2.97E-01 | |
| CDR3 Length (nt) | 42.77 (1.39) | 41.00 (1.30) | 41.56 (7.38) | 39.41 (2.85) | 2.58E-01 | 1.13E-01 | 2.44E-02 | 9.31E-01 | |
| IgG | Entropy (bit) | 6.80 (1.26) | 7.69 (0.73) | 2.22 (1.74) | 3.75 (1.82) | 2.31E-06 | 8.23E-05 | 2.52E-02 | 7.08E-02 |
| SHM (rate) | 0.0852 (0.0126) | 0.0745 (0.0102) | 2.28E-3 (7.39E-4) | 2.96E-3 (1.34E-3) | 1.92E-07 | 4.11E-05 | 1.86E-01 | 1.86E-01 | |
| CDR3 Length (nt) | 42.80 (1.73) | 42.36 (2.25) | 44.53 (5.22) | 42.32 (6.52) | 2.04E-01 | 6.05E-01 | 6.47E-01 | 1.26E-01 | |