Table 3. Summary of term maternal versus neonate analysis for TCR-β and IgH V, D and J gene segment usage.
In pregnancies carried to term, a substantial number of genes had significantly higher usage in cord blood (compared to maternal blood) or in maternal blood (compared to cord blood), across TCR and various IgH isotypes. The usage difference in fold-change is noted next to each gene.
| Variable (V) genes | Diversity (D) genes | Joining (J) genes | ||
|---|---|---|---|---|
| TCR | Higher cord blood usage | TRBV6-5 (1.42), TRBV6-6 (1.62), TRBV10-3 (1.31) | TRBD2 (1.07) | TRBJ2-3 (1.22), TRBJ2-4 (1.42) |
| Higher maternal blood usage | TRBV7-8 (1.56), TRBV7-9 (1.33), TRBV18 (1.64) | |||
| IgD unmutated | Higher cord blood usage | IGHV6-1 (2.71) | IGHD1-7 (1.74), IGHD1-26 (1.57), IGHD6-6 (1.93), IGHD7-27 (9.08) | IGHJ1 (1.47), IGHJ2 (2.49), IGHJ3 (1.88), IGHJ4 (1.09) |
| Higher maternal blood usage | IGHV3-48 (1.37) | IGHD3-16 (1.29), IGHD4-17 (1.59), IGHD6-19 (1.43) | IGHJ5 (1.35), IGHJ6 (1.55) | |
| IgD mutated | Higher cord blood usage | IGHD1-26 (1.55), IGHD6-6 (2.03), IGHD7-27 (5.97) | IGHJ2 (2.53), IGHJ3 (1.83) | |
| Higher maternal blood usage | IGHD2-15 (1.45), IGHD5-24 (1.53), IGHD6-19 (1.42) | IGHJ5 (1.46), IGHJ6 (1.48) | ||
| IgM unmutated | Higher cord blood usage | IGHV6-1 (2.81) | IGHD1-7 (1.59), IGHD1-26 (1.43), IGHD6-6 (1.93), IGHD6-13 (1.34), IGHD7-27 (7.52) | IGHJ2 (2.15), IGHJ3 (1.69) |
| Higher maternal blood usage | IGHV1-46 (1.43), IGHV3-21 (1.23) | IGHD2-15 (1.62), IGHD3-10 (1.29), IGHD4-17 (1.56), IGHD6-9 (1.42) | IGHJ5 (1.52), IGHJ6 (1.54) | |
| IgM mutated | Higher cord blood usage | IGHV1-3 (1.83) | IGHD1-26 (1.40), IGHD6-6 (2.07), IGHD6-13 (1.51), IGHD7-27 (5.68) | IGHJ2 (2.29), IGHJ3 (1.75) |
| Higher maternal blood usage | IGHV3-7 (1.60), IGHV3-48 (1.56), IGHV3-74 (1.92) | IGHD2-15 (1.56), IGHD3-16 (1.33), IGHD4-17 (1.49), IGHD6-19 (1.53), IGHD6-25 (1.62) | IGHJ4 (1.06), IGHJ5 (1.58), IGHJ6 (1.37) |