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(A) The structures of the sfGFP-PLpro fusion protein whose expression in E. coli have been tested. (B) Structures of two PLpro substrates and their catalytic release of AMC. (C) The catalytic release of AMC at various concentrations of Ub-AMC by 20 nM PLpro. (D) The catalytic release of AMC at various concentrations of Z-LRGG-AMC by 20 nM PLpro. (E) The catalytic release of AMC at 50 µM LRGG-AMC by 20 nM PLpro in the presence of various concentrations of DMSO.
