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(A) Initial screening of PLpro inhibition by 33 deubiquitinase inhibitors. (B) Initial screening of PLpro inhibition by 37 cysteine protease inhibitors. Reaction conditions: 20 nM PLpro, 50 μM Z-LRGG-AMC, 200 μM inhibitor, 20 mM Tris-HCl, 300 mM NaCl, pH 7.5.
