Table 2.

Reported input requirements of TSS mapping methods

Method	Enzymatic approach	Reported inputs
TSS-seq	Oligo-capping	200 μg total RNA (Yamashita et al., 2011); 500 ng poly(A)+ RNA (Malabat et al., 2015)
PEAT	Oligo-capping	1–2 μg poly(A)+ RNA (Ni et al., 2010); 30 μg total RNA (Morton et al., 2014)
CapSeq	Oligo-capping	500 ng–2 μg total RNA (Gu et al., 2012)
TL-seq	Oligo-capping	1 μg poly(A)+ RNA (Arribere and Gilbert, 2013)
TIF-seq	Oligo-capping	60 μg total RNA (Pelechano et al., 2013)
SMORE-seq	Oligo-capping	500 ng poly(A)+ RNA (Park et al., 2014)
nAnT-iCAGE	Cap-trapping	5 μg total RNA (Murata et al., 2014)
SLIC-CAGE	Cap-trapping	1–100 ng total RNA brought up to 5 μg with carrier (Cvetesic et al., 2018)
MAPCap	Cap-trapping	100 ng–5 μg total RNA (Bhardwaj et al., 2019)
nanoCAGE-XL	Template-switching reverse transcription	200 ng rRNA-depleted RNA (Cumbie et al., 2015); 7.5 μg total RNA (Adiconis et al., 2018)
nanoCAGE 2017	Template-switching reverse transcription	50–500 ng total RNA (Poulain et al., 2017); Single cell (C1 CAGE [Kouno et al., 2019])
RAMPAGE	Template-switching reverse transcription/cap-trapping	5 μg total RNA (Batut et al., 2013)
Tn5Prime	Template-switching reverse transcription	Single cell – 5 ng total RNA (Cole et al., 2018)
nanoPARE	Template-switching reverse transcription	10 pg (single-cell equivalent) – 5 ng total RNA (Schon etal., 2018)
STRIPE-seq	Template-switching reverse transcription	50–250 ng total RNA (Policastro et al., 2020)