Fig. 2.

MET silencing increases H₂O₂-induced apoptotic AoSMCs.

AoSMCs were transfected with scrambled siRNA (Scr) or MET-specific siRNA for 12 h in full medium, and then cultured in fresh medium until sub-confluency. The culture was continued in the presence of indicated concentrations of H₂O₂ for another 16 h, and then used for FACS assay (A), and the FCS Express 6 software was used. The assay was performed in a different time than that in Fig. 1 and 5. Quantification (B): Percent apoptotic cells vs total cells; mean ± SD; paired Student’s t-test, **P < 0.01, ***P < 0.001.