Fig. 3.

Smad2 cooperates with p53 in regulating MET expression.

A and B. Co-IP between P-Smad2 and p53. C and D. Activating p53 potentiates MET transcription inhibition in a Smad2-dependent manner. E. Activating p53 does not reduce Smad2. Human primary aortic SMCs (AoSMCs) were cultured, transfected, treated, and assayed as described for Fig. 1. Quantification was performed as described for Fig. 1; mean ± SD for mRNA levels in C (n = 3 repeats) and mean ± SEM for Western blots, n = 3 independent repeat experiments. Statistics: One-way ANOVA/Bonferroni post-hoc test; *P < 0.05, **P < 0.01, ***P < 0.001.