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. Author manuscript; available in PMC: 2010 May 25.
Published in final edited form as: J Neurosci. 2009 Nov 25;29(47):14903–14911. doi: 10.1523/JNEUROSCI.3436-09.2009

Figure 4. PP2A is required for D1R-dependent dephosphorylation of Cx36 at Ser293 in AII amacrine cells.

Figure 4

A & B, Control; color scheme and antibodies are the same as Figure 2. C & D, D1R activation (SKF38393, 10 µM) greatly diminished Ser293-P labeling. E & F, Inhibition of PP2A (microcystin-LR, 0.5 nM) completely blocked the reduction in Ser293 phosphorylation caused by D1R activation. G & H, Inhibition of PP2A alone led to increased phosphorylation of Ser293. I, Summary of data shows that inhibition of PP2A significantly blocked the reduction in Ser293 phosphorylation caused by D1R activation, and that PP2A inhibition alone significantly increased Ser293 phosphorylation. J, Summary of data shows that changes in the percentage of Cx36 plaques that show detectable Ser293-P labeling follows the same pattern established for relative Ser293-P measurements in I. Error bars are s.e.m, n = 6. Asterisk denotes P < 0.05, two asterisks denote P < 0.01. Images are 1 µm-deep stacks. Scale bar in H is 10 µm.