Table 3. mRNA fold changes in spinal cord of C57BL/6 mice injected with CFA, CFA + pertussis toxin (PTx) or PTx alone.

Mice were sacrificed at the indicated times post-injection with CFA, CFA + pertussis toxin, or pertussis toxin alone. Each determination employed 3 mice, and 3 determinations were made at each time-point. Data are presented as means of fold-differences over uninjected control mice. Shaded boxes indicate that mRNA levels were at least 1.5 fold higher, and significantly different, than in the control samples at that time-point, with a p<0.05 (Mann-Whitney U test).

	3 hours post - injection			24 hours post - injection			48 hours post - injection			72 hours post - injection			96 hours post - injection
	CFA	CFA+PTx	PTx	CFA	CFA+PTx	PTx	CFA	CFA+PTx	PTx	CFA	CFA+PTx	PTx	CFA	CFA+PTx	PTx
MPO	2.1	0.7	0.5	4.1	0.9	0.5	1.3	0.5	0.7	1.3	1.1	0.4	15.9	1.0	0.9
COX2	2.6	5.9	0.9	3.5	3.9	0.7	5.5	3.8	1.0	2.9	2.1	0.9	5.5	4.4	0.8
Mincle	1.0	2.2	0.4	1.8	2.1	0.4	2.4	1.6	0.6	1.4	1.5	0.3	4.0	4.0	0.6
IL1β	1.1	1.2	0.4	0.9	0.8	0.4	0.8	1.1	0.4	1.1	1.1	0.4	2.3	3.1	0.8
CXCL1	4.3	34.9	0.4	3.2	2.3	0.6	15.4	4.4	1.5	5.0	1.7	0.7	8.3	10.0	0.8
CD45	1.5	1.5	1.2	2.4	1.9	1.3	2.1	1.7	1.3	1.6	1.8	1.1	2.7	2.1	1.3

mRNAs tested but showing no change in spinal cords of mice given CFA, CFA+PTx, or PTx: HMGB1, NF-kb1, CX3CR1, CX3CL1, C5, NF-IL6, CXCL9