A) qPCR analysis of ATG7 and ATG12 expression after treatment with C-DIM12, ** p < 0.01, *** p < 0.001, B) Immunoblot analysis of ATG7 and ATG12 and Cleaved PARP in gemcitabine and C-DIM12 treated MiaPaCa2 cells, C) Immunoblot analysis of ATG7 and ATG12 in gemcitabine and C-DIM12 treated Panc1 cells. D/E. Immunoblot analysis of Cleaved PARP and p62 in the presence of si. ATG7 or si. ATG12 MiapaCa2 cells in the presence or absence of treatment with gemcitabine, C-DIM12, and combination of both. α-Tubulin serves as a loading control. F. Effects of ATG7 and ATG12 knockdown on GEM-induced survival of MiaPaca2 cells was determined as outlined in Figure 2F. Each data point represents the mean of 4 independent measurements.