Skip to main content
. Author manuscript; available in PMC: 2022 Oct 14.
Published in final edited form as: J Neuroendocrinol. 2021 Dec 5;33(12):e13063. doi: 10.1111/jne.13063

Figure 1: Validation of AR immunohistochemistry and Ar in situ hybridization probe.

Figure 1:

A-B, fluorescent images showing AR-immunoreactivity (AR-ir) in the adult female mouse brain (postnatal day/PND 56–70). AR-ir was observed in sections incubated in primary antibody (A), but not in sections without primary antibody (B). C-D, darkfield images showing silver grain deposition corresponding to Ar hybridization signal in adjacent sections from the same brain (PND 12 male mouse). Signal was observed in sections hybridized with an antisense probe (C), but not with a sense probe (D). Abbreviations: V3, third ventricle, PMv, ventral premammillary nucleus. Scale bar = 100 µm (A-B), 200 µm (C-D).