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. Author manuscript; available in PMC: 2022 Oct 14.
Published in final edited form as: J Neuroendocrinol. 2021 Dec 5;33(12):e13063. doi: 10.1111/jne.13063

Figure 5: Ar mRNA expression in cerebral nuclei of male and female prepubertal mice.

Figure 5:

Silver grain deposition corresponding to Ar mRNA hybridization signal in prepubertal (postnatal day (PND) 12 (A-B, G-H), and PND 21 (D-E, J-K) male (A, D, G, J) and female (B, E, H, K) mice. (A-F) Lateral septal nucleus, caudodorsal (LSc) and (G-L) bed nucleus of the stria terminalis, principal nucleus (BSTpr). Bar graphs showing mean ± SEM integrated optical density (IOD) of silver grains (C, F, I, L). IOD was analyzed by t-test with Welch’s correction for LSc male vs female PND 12 (P = 0.16, n = 7–8/sex), PND 21 (P = 0.96, n = 8/sex), BST male vs female PND 12 (P = 0.39, n = 5–7/sex), and BST male vs female PND 21 (P = 0.75, n = 8/sex). Abbreviations: cc, corpus callosum, LSr, lateral septal nucleus, rostral (rostroventral), MS, medial septal nucleus, PVT, paraventricular nucleus of the thalamus, RE, nucleus of reuniens, VL, lateral ventricle. Scale bar = 200 µm.