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. Author manuscript; available in PMC: 2022 Oct 14.
Published in final edited form as: J Neuroendocrinol. 2021 Dec 5;33(12):e13063. doi: 10.1111/jne.13063

Figure 7: Ar mRNA expression in hypothalamic nuclei of male and female prepubertal mice.

Figure 7:

Silver grain deposition corresponding to Ar mRNA hybridization signal in prepubertal (postnatal day (PND) 12 (A-B, I-J), and PND 21 (E-F, M-N) male (A, E, I, M) and female (B, F, J, N) mice. (A-H) Suprachiasmatic nucleus (SCH) and subparaventricular zone (SBPV), and (I-P) dorsal and ventral premammillary nuclei (PMd and PMv). Note higher expression of Ar in the SCH of males at PND 21 (E). Bar graphs showing mean ± SEM integrated optical density (IOD) of silver grains (C-D, G-H, K-L, O-P). IOD was analyzed by t-test with Welch’s correction for SCH male vs female PND 12 (P = 0.38, n = 5/sex), SCH male vs female PND 21 (P = 0.009, n = 6–7/sex), SBPV male vs female PND 21 (P = 0.45, n = 6–7/sex), PMv male vs female PND 21 (P = 0.21, n = 8–9/sex), PMd male vs female PND 12 (P = 0.58, n = 7–8/sex) and PND 21 (P = 0.19, n = 8–9/sex), and Mann-Whitney nonparametric test for SBPV male vs female PND 12 (P = 0.12, n = 6/sex), and PMv male vs female PND 12 (P = 0.57, n = 8/sex) Abbreviations: fx, fornix, V3, third ventricle. Scale bar = 200 µm.