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. Author manuscript; available in PMC: 2010 Jun 2.
Published in final edited form as: J Neurosci. 2009 Dec 2;29(48):15083. doi: 10.1523/JNEUROSCI.2782-09.2009

Figure 4. Effects of overexpression of twinfilin 2 on cochlear hair cells.

Figure 4

(A) Hair cells were transfected with a bicistronic expression vector for twinfilin 2 and EGFP (green). Shown is a representative pair of transfected and untransfected inner hair cells. F-actin was visualized with TRITC-conjugated phalloidin (red). Cochleae were dissected at P3-P4, transfected after 1 day in vitro (DIV) and images are taken at 2-3 DIV. (B) Control hair cell transfected with a vector expressing only EGFP (green). (C) Individual tall stereocilia lengths were averaged for each transfected hair cell and its untransfected neighbors and average length reductions are shown (mean ± SEM, n=9 for twinfilin 2, n=8 for controls). Statistical analysis with an unpaired one-tail T-test yields a significant change of p<0.0005. Scale bars = 5μm.