. Author manuscript; available in PMC: 2022 Dec 1.

Published in final edited form as: Cell Mol Life Sci. 2021 Nov 15;78(24):8283–8300. doi: 10.1007/s00018-021-04017-z

Table 1.

Basic properties of Col1a2^GFP clones selected for this study

Clone	Col1a2 genotype	Homotrimer secretion	Mineralization	Hspa5/Cryab/Ddit3 upregulation^†	SP7/Bglap expression
MC3T3	+/+	−	+	−	+
Col1a2^GFP-A	GFP/+	+	+	±	+
Col1a2^GFP-B	GFP/+	+	−	±	+
Col1a2^GFP-C	GFP/+	−	−	±	+
Col1a2^GFP-D	GFP/+	−	−	ND^‡	ND^‡
Col1a2^GFP-E	GFP/−	+	+	±	+
Col1a2^GFP-F	GFP/+	−	+	−	+

^†

The ± sign indicates a statistically significant yet relatively small (< 2 fold) increase in just one of these three markers of cell stress in osteoblasts (Hspa5 in clone E, Cryab in clones B,C, and Ddit3 in clone A).

^‡

ND – not determined. The cells had significantly altered morphology and were discarded after the mineralization experiment.