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. Author manuscript; available in PMC: 2010 Jun 16.
Published in final edited form as: J Neurosci. 2009 Dec 16;29(50):15770–15779. doi: 10.1523/JNEUROSCI.4951-09.2009

Figure 3. PSD-95/SAP102 knockdown diminishes spontaneous mEPSC frequency and PPD.

Figure 3

(A) Left: Whole-cell patch clamp recordings of mEPSCs in CG neurons in 1 μM TTX. Right: Overlay of 10 consecutive mEPSCs (gray) and the resulting mean (black) from the cells yielding the recordings. (B) Knockdown of PSD-95 and SAP102 (P95/S102-RNAi), but not SAP97 (S97-RNAi), decreased mean mEPSC frequency in CG neurons (*p ≤ 0.05 by ANOVA with Dunnet’s post-hoc test). (C) PSD-95/SAP102 knockdown also decreased mEPSC amplitude as seen in cumulative histograms (Cum Fraction; p ≤ 0.01 by K-S test; 9–15 cultures/condition). (D) Paired responses in patch-clamped CG neurons exhibited PPD when presynaptic inputs were stimulated (arrows) at an interval of 20 ms. (E) Neurons expressing PSD-95/SAP201-RNAi exhibited less PPD than did control neurons, whereas PPD was unchanged in neurons expressing SAP97-RNAi (*p ≤ 0.05 by unpaired Student’s t-test; 11–15 cultures/condition).