Figure 5.

Assessing Mmp14 expression across cell types in Sham and 4 day mechanical overloaded (4DMOV) muscles and macrophage sub clusters. (A) Under Sham conditions (red), FAPs are the predominant source of Mmp14. Following 4DMOV (blue) macrophages present as the majority of Mmp14 expressing cells. (B) Representative images of mouse skeletal muscle staining of pan macrophage marker CD206 (red), Mmp14 (green) and DAPI (blue) under Sham or 4DMOV conditions. Scale bar=20μm. (C) K-means clustering of macrophages from both sham and 4DMOV muscles reveals 3 sub clusters with Sham clustering separately (cluster 3, gold) and 4DMOV macrophage clusters consisting of cluster 1 (blue) and cluster 2 (red). (D) Feature plot of Mmp14 expression across the macrophage cluster. FAPs, fibro-adipogenic progenitors; MN, myonuclei; Endo, endothelial; Neutro, Neutrophils; Mmp14, matrix metalloproteinase 14; Macs, macrophages.