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. Author manuscript; available in PMC: 2010 Aug 10.
Published in final edited form as: J Neurosci. 2010 Feb 10;30(6):2365–2372. doi: 10.1523/JNEUROSCI.5462-09.2010

Figure 6.

Figure 6

The facilitatory effect of UTP on ATP-evoked sustained currents but not fast currents is mediated by the G protein-coupled P2Y2 receptor through a PKC-dependent pathway. Although the facilitatory effect of UTP on sustained currents was blocked by intracellular GDP-β-s (A), the increased fast currents induced by UTP/repeated ATP application were not inhibited by GDP-β-s (A and B). No significant change in either sustained or slow currents in response to repeated ATP application was found in bladder neurons containing GDP-β-s compared with controls (B). The myristoylated PKC inhibitor 20–28 (10μM) prevented the effect of UTP on the P2X2 sustained current in lumbosacral bladder neurons (C). No significant effect of UTP was observed on the slow current in the presence or absence of the PKC blocker in lumbosacral bladder neurons (C). Neurons from P2Y2 knockout mice did not show a facilitatory effect of UTP on sustained currents in lumbosacral bladder neurons compared with wild type bladder neurons (D).