Skip to main content
. Author manuscript; available in PMC: 2010 Aug 10.
Published in final edited form as: J Neurosci. 2010 Feb 10;30(6):2365–2372. doi: 10.1523/JNEUROSCI.5462-09.2010

Figure 7.

Figure 7

Single cell nested RT-PCR of P2X2, P2X3, P2Y2 and P2Y4 receptor subunits in lumbosacral (LS) and thoracolumbar (TL) bladder neurons. (A) Examples of positive nested single cell PCR amplification of P2X and P2Y mRNAs. Product length corresponded with expected size. The frequency of P2X and P2Y transcription in bladder neurons is summarized in (B). The P2X2 subunit transcript was significantly more abundant in LS than TL bladder neurons (P<0.01). The percentage of cells expressing P2Y2 and P2Y4 transcripts in P2X2- (C) or P2X3- (D) positive neurons is illustrated in pie charts.