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. Author manuscript; available in PMC: 2022 Oct 14.
Published in final edited form as: J Comp Neurol. 2022 Feb 21;530(10):1658–1699. doi: 10.1002/cne.25307

Figure 14. Atoh1 Cre fate-mapping with tdTomato.

Figure 14.

Cre fate-mapping for Atoh1 identified neurons in the cerebellum, PB, and several other brainstem regions, plus extensive labeling in fibrous processes. (a) Fluorescent reporter expression for Atoh1-Cre (tdTomato, pseudocolored coral-red) across five rostral-to-caudal tissue sections spanning the PB region. At each level, the approximate distance caudal to bregma is shown at bottom-right. (b–d) Cre fate-mapping for Atoh1 followed by immunofluorescence labeling for Lmx1b (ice blue). (e–h) Immunofluorescence labeling for Phox2b (green) and ChAT (blue) at a mid-level through the PB region (approximately bregma −5.1 mm). All scale bars are 200 μm. Scale bar in (b) also applies to panels (c–d). Scale bar in (h) also applies to panels (e–g).