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. Author manuscript; available in PMC: 2022 Oct 14.
Published in final edited form as: J Comp Neurol. 2022 Feb 21;530(10):1658–1699. doi: 10.1002/cne.25307

Figure 4. Immunofluorescence labeling for Lmx1b and FoxP2 protein.

Figure 4.

Combined immunolabeling for the nuclear transcription factors Lmx1b (red) and FoxP2 (green) identified largely separate distributions across successive, rostral-to-caudal sections through the PB region (a–f). Approximate level caudal to bregma is shown at the bottom-right of each panel (in mm). Lmx1b immunofluorescence labeling was mutually exclusive with FoxP2, except in a rostral cluster of neurons ventral to the PB (arrowhead in a) and in sparse, double-labeled neurons extending back to mid-rostral levels of the PB along the lateral edge of the brainstem (arrowheads in c). Scale bar in (a) is 200 μm and applies to panels (b-f). Other abbreviations: Bar, Barrington’s nucleus; DLL, dorsal nucleus of the lateral lemniscus.