Table 2.
Antisera.
| Antigen | Immunogen description | Source, Host Species, RRID | Concentration |
|---|---|---|---|
| Choline acetyltransferase (ChAT) | Human placental choline acetyltransferase | Millipore, goat polyclonal, #AB144P, lot: JC1618187; RRID: AB_2079751 | 1:1,000 |
| Forkhead box protein 2 (FoxP2) | Recombinant human FOXP2 isoform 1 Ala640-Glu715 | R&D Systems, sheep polyclonal #AF5647; RRID: AB_2107133 | 1:10,000 |
| LIM homeobox transcription factor 1 beta (Lmx1b) in rat | Three fusion proteins containing amino acid residues 17–47, 157–195, or 255–324 of mouse Lmx1b | Y. Ding, Chinese Academy of Science, Shanghai; rabbit polyclonal; RRID: AB_2314751 (no labeling in Lmx1b KO mice; Dai et al J Comp Neurol 2008) | 1:1,000 |
| Lmx1b (mouse) | Full-length LIM homeobox transcription factor 1 beta protein from mouse | C. Birchmeier, Max Delbruck Center for Molecular Medicine, Berlin; guinea pig polyclonal; RRID: AB_2314752 | 1:8,000 |
| mCherry | Full-length mCherry fluorescent protein | Life Sciences, rat monoclonal, #M11217, lot: R1240561; RRID: AB_2536611 (we find no immunolabeling in mice without dsRed-derived fluorescent proteins) | 1:2,000 |
| Paired-like homeobox 2b (Phox2b) | BSA-coupled 15mer corresponding to the C terminus of the Phox2b protein with an added N-terminal tyrosine | H. Enomoto, School of Medicine at Kobe University, Japan; RRID: AB_2895590 | 1:12,000 |
| Paired-like homeobox 2b (Phox2b) | Mouse monoclonal antibody raised against amino acids 11–70 mapping near the N-terminus Phox2b of human origin | Santa Cruz, mouse monoclonal, #sc-376997, lot: E1719; RRID: AB_2813765 | 1:1,000 |
| Special AT-rich sequence-binding protein 2 (Satb2) | Synthetic peptide within human SATB2 (proprietary sequence) | Abcam, rabbit monoclonal, cat. # ab92446, lot: GR325015–2, RRID: AB_1056367 | 1:3,000 |
| Tyrosine hydroxylase (TH) | Purified, SDS-denatured rat pheochromocytoma TH | Millipore, mouse monoclonal, #MAB318, lot: NG1802536; RRID: AB_2201528 | 1:2,000 |
| Tyrosine hydroxylase (TH) | Denatured TH from rat pheochromocytoma (denatured by sodium dodecyl sulfate | Millipore, rabbit polyclonal, #AB152, lot: 240602; RRID: AB_696697 | 1:10,000 |