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. Author manuscript; available in PMC: 2022 Mar 10.
Published in final edited form as: Nature. 2021 Dec 23;602(7897):487–495. doi: 10.1038/s41586-021-04352-y

Extended Data Fig. 8. Examples of leader-containing reads for Orf9b and N from RNAseq dataset.

Extended Data Fig. 8.

a, b, c. Representative sequence for Orf9b (top) and N (bottom) sgRNA from Alpha (a), VIC (b) and IC19 (c). Leader sequences to identify sgRNAs are highlighted in yellow. The following sequence is used to differentiate Orf9b versus N sgRNAs. Orf9b and N start codons shown in maroon. The site of the N-protein D3L mutation is indicated in green, resulting in increased similarity to the transcriptional regulatory sequence (TRS) for Alpha. Read counts of Orf9b and N are indicated to the right. Counts are normalized to mean genomic reads per replicate.