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. Author manuscript; available in PMC: 2022 Aug 31.
Published in final edited form as: Prostate. 2022 Feb 28;82(7):836–849. doi: 10.1002/pros.24326

Figure 4: Varying tissue digestion enzymes impacts cell yield, viability, and cellular populations.

Figure 4:

(A) Scatter plot displays mean cellular yields, with standard deviation, from individual prostate biopsies digested using numerous tissue digestion conditions. Digestion conditions A, B, C, D, and E are listed in the corresponding table (* indicates p-value <0.05, *** indicates p-value <0.001). (B) Scatter plot displays mean cell viability, with standard deviation, from individual prostate biopsies with and without 0.1% dispase added to digestion buffers (*** indicates p-value <0.001). (C) q-PCR measures androgen receptor transcripts (AR4/5, KLK3, FOLH1, TMPRSS2) and immune cell activation transcripts (IL-6, IL-1B, IL-8) from cells for both 10-minute digestion protocols and 4-hour digestion protocols. Fold change values are normalized to undigested tissue and averaged, n=3. (D) Gated flow cytometry dot plots display cell populations present in digested prostate samples from patient PB054 biopsies, with and without 0.1% dispase added (top row and bottom row respectively). The first column represents the live/single cell content of the isolates when is then projected to show CD45 expression in the second column. The third column represents EpCAM and CD49a expression within the CD45- fraction. The fourth and fifth columns show CD11b expression within the CD45+ subset and CD4/CD8 expression within the CD45+/CD11b- gate, respectively. Data shows frequency within the parent gates.