A) Genetic landscape comparison between RSPOfp vs. RNF43-mut tumors in the MSS subgroup. KRAS mutation was the only statistically different genetic alteration. ***q<0.001.
B) TMB and PDL-1 status in RNF43-mut, RSPOfp and RNF43/RSPOfp-WT CRCs. None of the RSPOfp patients showed a TMB >10 mt/Mb or a MSI-H/dMMR status.*q<0.05, **q<0.01, ***q<0.001.
C) Copy number alterations in RNF43 mutations, RSPO rearrangements and RNF43/RSPO-WT samples. *q<0.05.