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Comparison of macrocyclic hits with corresponding linear analogues. (A) A set of macrocyclic and linear compounds was resynthesized on-resin, incubated with 10 nM SA-A647, then assayed by FACS for SA binding. Higher RFU suggests a stronger binding affinity for the macrocyclic compounds. (B) Fluorescence polarization data indicating the binding affinities of two purified macrocyclic compounds and their linear analogues.
