TABLE 2.

Wnt-related genes whose promoters (3000 bp upstream to 300 bp downstream of TSS) overlap with H3K27me2/3 peaks in Klotho-stimulated C2C12 myoblasts

Symbol	Chr:Start-End	Gene ID	H3K272/3 peak(s) in KL-treated cells	Promotor overlap (%)
Wnt4	chr4:137277489–137299726	ENSMUSG00000036856	chr4:137274634–137274877; chr4:137276224–137277069	33
Wnt9a	chr11:59306928–59333552	ENSMUSG00000000126	chr11:59304349–59305664	40
Wnt10a	chr1:74791516–74804179	ENSMUSG00000026167	chr1:74789946–74790493	17
Fzd3	chr14:65201026–65262463	ENSMUSG00000007989	chr14:65261701–65263591	43
Fzd9	chr5:135248938–135251230	ENSMUSG00000049551	chr5:135252831–135253153	10
Frzb	chr2:80411970–80447625	ENSMUSG00000027004	chr2:80446165–80447428	3
Wisp3	chr10:39150971–39163794	ENSMUSG00000062074	chr10:39164763–39165022; chr10:39166089–39167811	29
Csnk2a2	chr8:95446096–95488820	ENSMUSG00000046707	chr8:95491364–95492299	14
Pp2cb	chr8:33599621–33619794	ENSMUSG00000009630	chr8:33597564–33598858	39
Sfrp2	chr3:83766321–83774316	ENSMUSG00000027996	chr3:83766030–83766774	18
Csnk1e	chr15:79417856–79443919	ENSMUSG00000022433	chr15:79444679–79446189	46
Nkd2	chr13:73818534–73847631	ENSMUSG00000021567	chr13:73848780–73850045	38
Nkd1	chr8:88521344–88594887	ENSMUSG00000031661	chr8:88520969–88524273	20
Shisa2	chr14:59625281–59631658	ENSMUSG00000044461	chr14:59625308–59626307	8

Note: Promoter overlap percentage indicates the percent of a given promoter overlapping with H3K27me2/3 peak(s) in the Klotho-treated condition. Notably, all listed promoters have 0% overlap with H3K27me2/3 peaks in the vehicle-treated control condition, which suggests that these Wnt-related promoters have preferential heterochromatic mark deposition under Klotho stimulation. Column names indicate: gene symbol, gene location (chr:start-end), Ensemble gene ID, location of broad peaks, and promotor overlap (%) with H3K27me2/3 peaks in C2C12 cells treated with recombinant Klotho for 48 h.