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. Author manuscript; available in PMC: 2023 Apr 12.
Published in final edited form as: Thorax. 2022 Mar 30;78(4):383–393. doi: 10.1136/thoraxjnl-2021-217526

Fig. 5. High protein level of PRMT4 causes lymphocyte death.

Fig. 5.

A, B. FACS analysis of apoptosis in PRMT4 KO or overexpressed Jurkat cells with or without LPS treatment. Data of A were quantitated in B. C. Lenti-PRMT4 or shRNA particles were delivered intratracheally into the mouse. Mouse splenic T cells were isolated and treated with LPS for 18 h, viable cells were counted. “Vec” denotes vector; “OE” denotes PRMT4 overexpression; “sh” denotes PRMT4 shRNA. D. Jurkat cells were treated with LPS and a range of PRMT4 inhibitors as indicated for 3 h. Cell lysates were analyzed for cleaved caspase 3. Relative expression of cleaved caspase 3 in each group were plotted in the lower panel. Independent experiments n=3. E. Isolated mouse splenic T cells were treated with LPS and TP064, cleaved caspase 3 was immunoblotting analyzed and plotted in the lower panel. Independent experiments n=3. “*” denotes p=0.05-0.01, “**” denotes p=0.01-0.001, “***” denotes p=0.001-0.0001.