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. Author manuscript; available in PMC: 2010 Aug 17.
Published in final edited form as: J Neurosci. 2010 Feb 17;30(7):2504–2512. doi: 10.1523/JNEUROSCI.3262-09.2010

Figure 3.

Figure 3

NPF suppression of sugar excitation of NPFR1-positve PAIN neurons from postfeeding larvae Stimulation paradigm: the tissue was initially perfused with HL6-lactose solution for 5 min before imaging. Each tissue was imaged in HL6-Lactose for 60 sec, and then in HL6-Fructose for 120 sec. (A) Representative traces showing G-CaMP fluorescence changes of thoracic PAIN neurons of normal postfeeding larvae (96h AEL) in response to fructose. The numbers on the traces correspond to the labeled neurons in panel B. (B) G-CaMP labeled PAIN neurons in the second thoracic segment. (C) Quantification of the NPF inhibitory effect on sugar excitation of thoracic clusters of PAIN neurons. Perfusion of larval tissues with 1 µM NPF significantly attenuated the number of G-Camp-expressing PAIN neurons excitable by fructose.