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. Author manuscript; available in PMC: 2022 Oct 14.
Published in final edited form as: J Cell Physiol. 2022 May 14;237(7):3012–3029. doi: 10.1002/jcp.30769

Figure 5: Effect of AdCLU on the levels and distribution ECM and pro-fibrotic proteins.

Figure 5:

Protein expression analysis by western blot showed significant decrease in - A. ECM proteins- COL1A, FN, ELN and pro-fibrotic TGFβ2 and TSP-1 in WCL, B. secretory FN and pro-fibrotic PAI-1 and TSP-1 in CM showed - in AdCLU compared to AdMT. GAPDH was used as a loading control. Values represent the mean ± SEM, where n=4 (biological replicates). *p≤0.05 was considered significant. D. Immunofluorescence imaging shows increase in clusterin distribution in AdCLU treatment (Alexa Flour 568/red) (first column in bottom panel) and a concomitant reduction in actin stress fiber distribution stained by phalloidin (green) (second column in bottom panel) compared to AdMT (top panel). AdCLU treatment reduced COL1A (Alexa Flour 568/red) (second column bottom panel), and FN (Alexa Flour 488/green) (third column bottom panel) distribution compared to AdMT. The nucleus was stained with DAPI in blue. Images were captured in z-stack in a confocal microscope, and stacks were orthogonally projected. Scale bar 50 microns.