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. Author manuscript; available in PMC: 2022 Oct 14.
Published in final edited form as: J Cell Physiol. 2022 May 14;237(7):3012–3029. doi: 10.1002/jcp.30769

Figure 6: Clusterin regulates ECM remodeling by MMP activation.

Figure 6:

Serum-starved HTM cells transduced with AdCLU compared to AdMT shows – A. no change in MMP2 mRNA expression, B. no change on MMP2 protein expression. GAPDH was used as a loading control. C. significant decrease in mRNA expression of tissue inhibitors of metalloproteinases (TIMP1 & TIMP2). D and E. significant increase in active form of MMP2 (62 kDa) as visualized by gelatin zymography. No significant change is seen in pro-MMP2 (72 kDa). MMP1, and MMP-9 were detected. Values represent the mean ± SEM, where n=3–4 (biological replicates). *p≤0.05 was considered significant.