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. Author manuscript; available in PMC: 2023 Jan 5.
Published in final edited form as: Clin Cancer Res. 2022 Jul 15;28(14):3076–3090. doi: 10.1158/1078-0432.CCR-22-0486

Figure 4 – Anti-VEGFR2 antibody treatment at 40 mg/kg transiently increases interstitial pO2 on day 2 and restores perfusion in MCaIV.

Figure 4 –

(A) Representative images of angiography of murine mammary carcinoma (MCaIV) tumors treated with control IgG (left panel) and murine anti-VEGFR2 antibody DC101 (right panel) (both 40 mg/kg i.p. every 3 days) on day 5 after two cycles of treatment. Tumor vasculature is presented as a colorized maximum intensity depth projection (superficial to deep: green, red, white). Scale bars, 500 μm. (B) Measurements of oxygen tension in mmHg in vessels in control IgG and DC101 antibody treated mice at baseline, day 2 and day 5. n = 81 – 226 measurements per timepoint per group. N = 4 mice per group. Horizontal black lines indicate statistically significant differences between multiple comparisons after a Kruskal-Wallis test with Dunn’s correction, with differences only depicted for comparisons between control and treatment groups on the same day. (C) Extravascular (interstitial, i.e., measurements 40 to 60 μm from the nearest vessel) oxygen measurements in the same tumors. n = 138 – 328 measurements per timepoint per group. (D) Measurements of extravascular oxygen tension in mmHg normalized to the intravascular oxygen tension of blood vessels. (E) Distance to the nearest vessel measurements in the same tumors. n = 1,117 – 1,851 measurements per group.