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. Author manuscript; available in PMC: 2022 Jun 6.
Published in final edited form as: Sci Immunol. 2022 Aug 26;7(74):eabo6294. doi: 10.1126/sciimmunol.abo6294

Fig. 5. Zα domain of ZBP1 drives PANoptosis mediated by IFN-β during β-coronavirus infection.

Fig. 5.

(A–C) Immunoblot analysis of (A) pro- (P53) and activated (P30) gasdermin D (GSDMD), pro- (P53) and activated (P34) gasdermin E (GSDME); (B) pro- (P55) and cleaved caspase-8 (CASP8; P18), pro- (P35) and cleaved caspase-3 (CASP3; P19 and P17) and pro- (P35) and cleaved caspase-7 (CASP7; P20); and (C) phosphorylated MLKL (pMLKL), total MLKL (tMLKL), phosphorylated RIPK3 (pRIPK3) and total RIPK3 (tRIPK3) in the lung samples from PBS-treated mice or mouse hepatitis virus (MHV)-infected wild type (WT) and Zbp1−/− mice treated with IFN-β harvested 3 days after infection. (D–F) Immunoblot analysis of (D) pro- (P45) and activated (P20) caspase-1 (CASP1), pro- (P53) and activated (P30) GSDMD, pro- (P53) and activated (P34) GSDME; (E) pro- (P55) and cleaved (P18) CASP8, pro- (P35) and cleaved (P19 and P17) CASP3 and pro- (P35) and cleaved (P20) CASP7; and (F) pMLKL, tMLKL and ZBP1 in PBS- or IFN-β–treated WT, Zbp1−/− and Zbp1ΔZa2 bone marrow-derived macrophages (BMDMs) during MHV infection. Actin was used as the internal control. Data are representative of at least three independent experiments.