KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-Spike of SARS-CoV-2 | Sino biological | MA14AP0204 |
| AlexaFluor 488 Goat anti-rabbit | Thermo Fisher | Cat # A-11034 |
| Anti-FLAG M2 agarose affinity gel (For IP) | Sigma-Aldrich | A2220 |
| Anti-acety-lysine (For IP) | ImmuneChem | ICP0380 |
| Anti-FLAG M2 HRP conjugated (For WB) | Sigma-Aldrich | A8592 |
| Anti-Brd4 (For WB) | Abcam | Ab46199 |
| Bacterial and Virus Strains | ||
| Escherichia coli BL21-CodonPlus (De3) RIL | Agilent Technologies | 230245 |
| DH5α Competent Cells | Thermo Fisher Sci | Cat. # 18265017 |
| SARS-Related Coronavirus 2, Isolate hCoV-19/USA/MD-HP05647/2021 | BEI Resources, NIAID, NIH | NR-55672 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Dithiothreitol | Gold Biotechnology | 27565-41-9 |
| β-mercaptoethanol | Sigma-Aldrich | Cat. # M6250 |
| Imidazole | Alfa Aesar | Cat. # A10221 |
| 15NH4Cl | Sigma-Aldrich | Cat. # 299251 |
| JQ1 | Cayman Chemical | 11187 |
| OTX015 | Cayman Chemical | 15947 |
| IPTG | Goldbio | Cat. # I2481 |
| Ni-NTA resin | Thermo Scientific | Cat. # 88223 |
| Glutathione Sepharose 4B beads | GE Healthcare | Cat. # 17-0756-01 |
| Streptavidin magnetic beads | Thermo Fisher Sci | Cat. # 88816 |
| E peptides | Synpeptide | N/A |
| PreScission protease | Home expressed | N/A |
| Thrombin protease | MP Biomedicals | 154163 |
| Enterokinase protease | NEB | P8070S |
| Hoechst 33258 | Invitrogen | Cat # H3570 |
| Cell Mask | Invitrogen | Cat # C10046 |
| Recombinant p300 (catalytic domain) | Active Motif | 81093 |
| Lipofectamine 3000 | Invitrogen | L3000001 |
| Critical Commercial Assays | ||
| Platinum™ II Hot-Start PCR Master Mix (2X) | Thermo Fisher Sci | 14000013 |
| QIAprep Spin Miniprep Kit | QIAGEN | Cat # 27106 |
| Deposited Data | ||
| Crystal structure of BRD4 Bromodomain 1 in complex with SARS-CoV-2 E peptide diacetylated at K53 and K63 | This study | PDB: 7TV0 |
| Crystal structure of BRD4 Bromodomain 1 in complex with SARS-CoV-2 E peptide acetylated at K63 | This study | PDB: 7TUQ |
| Experimental Models: Cell Lines | ||
| Calu-3 cells | ATCC, Manassas, VA, USA | HTB-55 |
| K562 | ATCC | CCL-243 |
| 293T | ATCC | CRL-3216 |
| Oligonucleotides | ||
| BamH1 E: atataggatccTACAGCTTCGTATCAGAAGA | This study | N/A |
| Xho1 E: atatactcgagTCATTCGAGAACGAGGAGATC | This study | N/A |
| gBlockE: TACAGCTTCGTATCAGAAGAAACCGGGACACTGATCGTAAATTCTGTGCTCTTGTTTCTGGCATTCGTCGTATTTCTCCTCGTCACACTGGCAATTCTGACTGCATTGAGGCTTTGCGCCTACTGTTGTAACATTGTCAATGTATCTCTCGTGAAACCCTCATTCTACGTTTACAGCAGGGTGAAGAATCTCAATTCTAGCAGGGTGCCGGATCTCCTCGTTCTCGAA | This study | N/A |
| E_Sarbeco_F1: ACAGGTACGTTAATAGTTAATAGCGT | This study | N/A |
| E_Sarbeco_R2: ATATTGCAGCAGTACGCACACA | This study | N/A |
| E_Sarbeco_P1:FAM -ACACTAGCCATCCTTACTGCGCTTCG - BHQ1 | This study | N/A |
| Recombinant DNA | ||
| pGEX-6P-1 | Cytiva | 28-9546-48 |
| pGEX-4T-1 | Cytiva | 28-9545-49 |
| pcDNA3.1 | Invitrogen | V79020 |
| Software and Algorithms | ||
| XDS | Kabsch, 2010 | https://xds.mr.mpg.de/ |
| Phenix | Adams et al., 2010 | http://www.phenix-online.org/ |
| Coot | Emsley et al., 2010 | https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/ |
| Prism 9.0 | GraphPad | https://www.graphpad.com/scientific-software/prism/ |
| CCP4 | M. D. Winn et al., 2011 | https://www.ccp4.ac.uk/ |
| NMRPipe | Delaglio et al., 1995 | https://www.ibbr.umd.edu/nmrpipe/ |
| MO.Affinity Analysis | NanoTemper Technologies | https://nanotempertech.com/monolith-mo-control-software/ |
| MO.Control software | NanoTemper Technologies | https://nanotempertech.com/monolith-mo-control-software/ |
| Other | ||
| Quantitative PCR (qPCR) Control RNA from Heat-Inactivated SARS-Related Coronavirus 2, Isolate USA-WA1/2020 | BEI Resources, NIAID, NIH | NR-52347 |
| HiPrep™ 16/60 Sephacryl® S-100 HR column | GE Healthcare | Cat. # 17-1165-01 |
| HiTrap SP HP column | Cytiva | 17115201 |
| HiLoad Superdex 75 pg column | Cytiva | 28989333 |