Skip to main content
. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: J Gen Virol. 2009 Dec 16;91(Pt 5):1284–1289. doi: 10.1099/vir.0.018143-0

Table 3.

Growth properties in mice of mutant VD5 viruses possessing human-like amino acids.

Virus Days post-infection Virus Titer (mean log10 PFU ± SD/g) in: *
Lung Nasal turbinates Brain
VD5 1 - - -
3 - - -
VD5K 1 4.7 ± 0.9 - -
3 7.0 ± 0.4 3.0 ± 0.7 -
VD5K-PB2-9N 1 4.9 ± 0.5 - -
3 7.0 ± 0.2 2.9, 6.0 -
VD5K-PB2-199S 1 4.8 ± 0.3 - -
3 7.1 ± 0.3 2.2 -
VD5K-PB2-368K 1 3.9 ± 0.3 - -
3 6.9 ± 0.6 3.9 -
VD5K-PA-142E 1 7.0 ± 0.3 2.3 -
3 7.5 ± 0.1 3.7, 4.4 1.4
VD5K-PA-142N 1 5.7 ± 0.5 - -
3 7.2 ± 0.4 5.7, 5.2 -
VD5K-PA-421I 1 4.7 ± 0.4 - -
3 6.8 ± 0.1 - -
VD5K-NP-33I 1 4.0 ± 0.3 - -
3 6.3 ± 0.1 4.8 -
VD5K-NP-357K 1 4.6 ± 0.1 - -
3 6.2 ± 0.2 2.2 -
*

Mice (3 per group) were infected intranasally with 100 PFU of virus. Organs were collected from infected mice on days 1 and 3 post-infection for virus titration. Individual titers were recorded when virus was not recovered from all three animals.

The virus titer in lungs of mice infected with VD5K-PA-142E was significantly higher than that of VD5K-infected mice on day 1 post-infection as determined by Student’s t-test (p<0.02).

-, Virus was not recovered (detection limit for lung and nasal turbinates is < 1.6 log10 PFU/g and for brain is <1.1 log10 PFU/g).