Table 3.
Virus | Days post-infection | Virus Titer (mean log10 PFU ± SD/g) in: * | ||
---|---|---|---|---|
Lung | Nasal turbinates | Brain | ||
VD5 | 1 | - | - | - |
3 | - | - | - | |
VD5K | 1 | 4.7 ± 0.9 | - | - |
3 | 7.0 ± 0.4 | 3.0 ± 0.7 | - | |
VD5K-PB2-9N | 1 | 4.9 ± 0.5 | - | - |
3 | 7.0 ± 0.2 | 2.9, 6.0 | - | |
VD5K-PB2-199S | 1 | 4.8 ± 0.3 | - | - |
3 | 7.1 ± 0.3 | 2.2 | - | |
VD5K-PB2-368K | 1 | 3.9 ± 0.3 | - | - |
3 | 6.9 ± 0.6 | 3.9 | - | |
VD5K-PA-142E | 1 | 7.0 ± 0.3† | 2.3 | - |
3 | 7.5 ± 0.1 | 3.7, 4.4 | 1.4 | |
VD5K-PA-142N | 1 | 5.7 ± 0.5 | - | - |
3 | 7.2 ± 0.4 | 5.7, 5.2 | - | |
VD5K-PA-421I | 1 | 4.7 ± 0.4 | - | - |
3 | 6.8 ± 0.1 | - | - | |
VD5K-NP-33I | 1 | 4.0 ± 0.3 | - | - |
3 | 6.3 ± 0.1 | 4.8 | - | |
VD5K-NP-357K | 1 | 4.6 ± 0.1 | - | - |
3 | 6.2 ± 0.2 | 2.2 | - |
Mice (3 per group) were infected intranasally with 100 PFU of virus. Organs were collected from infected mice on days 1 and 3 post-infection for virus titration. Individual titers were recorded when virus was not recovered from all three animals.
The virus titer in lungs of mice infected with VD5K-PA-142E was significantly higher than that of VD5K-infected mice on day 1 post-infection as determined by Student’s t-test (p<0.02).
-, Virus was not recovered (detection limit for lung and nasal turbinates is < 1.6 log10 PFU/g and for brain is <1.1 log10 PFU/g).