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. Author manuscript; available in PMC: 2022 Jun 26.
Published in final edited form as: Mol Microbiol. 2022 May 28;117(6):1464–1478. doi: 10.1111/mmi.14921

Figure 2: The effect of mutations to SHP144 on transcriptional activation by Rgg144 using a LacZ reporter assay.

Figure 2:

The reporter strain carrying a lacZ under the putative promoter of shp144, was genetically complemented with either wild-type (WT) or mutant shp144 in a Δshp144 background. Strains were grown in chemically-defined medium supplemented with 55 mM mannose to late exponential phase. Rgg-dependent transcription from the Pshp144 (where P is promoter) was measured by β-galactosidase activity relative to Pshp144∷lacZ-Δshp144com, which expresses wild type shp144 (WT). The sequence of the 13-residue long active SHP144 representing the C-terminal end is shown on top of the bar chart, and the numbers are relative to the full length SHP144. ** p<0.01, **** p<0.0001, ‘ns’ non- significant compared to the WT. The error bars represent the standard error of the mean for at least three independent replicates.