Table 1:
Transcription activation and binding capabilities of native and modified SHPs
| Peptidea | Transcription activationb (Miller units/109 cells) |
Binding affinities (KDc in μM) |
|---|---|---|
| C13 (WT) | 215±5 | 6.6±0.3 |
| S14A | 105±5 | 8.1±0.9 |
| E15A | 107±2.5 | 3.3±0.1 |
| W16A | 7.5±2.5 | 50.0±3.6 |
| V17A | 6±1 | 9.7±0.6 |
| I18A | 7.5±1.5 | >100 |
| V19A | 215±5 | 4.0±0.8 |
| I20A | 8.5±0.5 | 23.54 |
| P21A | 7.5±2.5 | 1.7±0.1 |
| F22A | 90±10 | 7.1±0.4 |
| L23A | 95±5 | 4.3±0.2 |
| T24A | 175±5 | 3.3±0.1 |
| N25A | 185±5 | 3.3±0.2 |
| L26A | 182±2.5 | 3.4±0.3 |
a
WT C13 or modified peptides were used. The numbers show the position of alanine replacement within the full length Shp144.
b
mean ± SEM of three independent experiments
c
Dissociation constant between the Rgg144 and its ligand SHP144