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. Author manuscript; available in PMC: 2022 Dec 30.
Published in final edited form as: Am J Transplant. 2022 Jun 15;22(10):2306–2322. doi: 10.1111/ajt.17116

Figure 1. MCMV induces intragraft infiltration of Th17 cells co-expressing Th1 cytokines.

Figure 1.

(A) Study design. Renal transplantation was performed between BALB/c and C57BL/6 mice with cyclosporine A (CsA) subcutaneous (SC) injections given daily for post-transplant immunosuppression. On day 7, splenocytes and intragraft leukocytes were analyzed by flow cytometry. (B) Representative flow cytometry plots showing frequencies of CD4+IL-17A+ (Th17) cells and cytokine expression profiles. (C) Frequencies of cytokine-expressing Th17 cell subsets were compared for D−R− and D+R+ allografts. (D) Intragraft quantities of IL-17A, IFN-γ and TNF-α (picogram per gram tissue, pg/g) in D−R− and D+R+ allografts. (E) Frequencies of IL-17A expressing CD4+, CD8+ and MHC-II+ leukocytes in D−R− and D+R+ allografts. (F) Representative flow plots and frequencies of Th17 cells in D+R+ spleens (SPLN) and allografts (GRAFT). (G) Cytokine quantities (pg/g) in D+R+ spleens and allografts. All data are represented as mean ± standard deviation (SD) and analyzed by two- sided Student’s t-test. NS, not significant (p>0.05).