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. Author manuscript; available in PMC: 2022 Dec 30.
Published in final edited form as: Am J Transplant. 2022 Jun 15;22(10):2306–2322. doi: 10.1111/ajt.17116

Figure 7. Depletion of Tregs and Th1 cells does not change allograft damage in the presence of Th17 cells.

Figure 7.

(A) D+R+ recipients were treated with αCD25 antibodies or isotype control antibodies at days 1 (400μg) and 4 (200μg) post-transplantation and sacrificed at day 7. (B) Representative flow plots and summary data showing frequencies of Foxp3+ Tregs between αCD25 treated mice and isotype controls. (C-D) Representative flow plots and summary data showing the frequencies of Th17 and Th1/17 cells between the groups. (E-F) Representative flow plots and summary data showing the frequencies of total (PMA+) and MCMV specific (Peptide+) Th1 cells in allografts (E) and spleens (F) between the groups. (G) H&E-stained allografts from αCD25 treated and control groups (40x). Scale bar 20μm. (H) Damage score. All data are represented as mean ± standard deviation (SD) and are analyzed by two- sided Student’s t-test and ANOVA. NS, not significant (p>0.05).