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. Author manuscript; available in PMC: 2010 Aug 24.
Published in final edited form as: J Neurosci. 2010 Feb 24;30(8):2967–2978. doi: 10.1523/JNEUROSCI.5552-09.2010

Figure 3. Effects of NAD+ on PARP-1 - induced neuronal death.

Figure 3

(A–C) Neuronal death was induced by MNNG (A,D,G) NMDA (B,E,H), and chemical ischemia (C,F,I). Incubation times were 30 minutes for MNNG, 10 minutes for NMDA, and variable with CI. Dose-response curves are shown for each condition (A–C). NMDA-induced neuronal death was blocked by 10 µM MK801 as a positive control. Neuronal death in all 3 conditions was reduced by co-incubation with the PARP inhibitor, DPQ (10 µM). ** p<0.01 vs. control (wash only); n = 3 −4.

(D–F) Panels D, E, and F show the effects of post-treatment with 1 – 10 mM NAD+ on neuronal death under each of the conditions shown in A–C. The NAD+ was added after wash-out of MNNG, CI, or NMDA. ** p<0.01 vs. no added NAD+; n = 3 −4.

(G–I) Panels G, H, and I show the effects of 5 mM NAD+ or 10µM DPQ. on neuronal survival when added to the culture medium at increasing time intervals after wash-out of MNNG, CI, or NMDA. ** p<0.01 vs. no added NAD+; n = 3 −4.