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. Author manuscript; available in PMC: 2010 Aug 24.
Published in final edited form as: J Neurosci. 2010 Feb 24;30(8):2967–2978. doi: 10.1523/JNEUROSCI.5552-09.2010

Figure 4. PARP-1 activation causes a block in neuronal glycolysis that is reversed by NAD+.

Figure 4

Neurons were treated with 75 µM MNNG for 30 minutes or 2.5 mM SIN1 for 60 minutes. Where used, PJ34 (200 nM) and DPQ (10 µM) were added with MNNG or SIN1.

(A,B) Glycolytic rate and pyruvate content were measured 1h after MNNG or SIN1 washout. n = 3; ** p < 0.001 vs. control.

(C,D) 5 mm NAD+ or 2.5 mM pyruvate was added to the after washout of MNNG or SIN1. Glycolytic rate was restored by NAD+, but not pyruvate, whereas intracellular pyruvate concentrations were restored by both NAD+, but not pyruvate. PJ34 or DPQ were given simultaneously with MNNG or SIN1. n =3, ** p < 0.001 vs. no post-treatment (white bar).

(E,F) ATP and AMP were measured 1h after MNNG or SIN1 washout. The effects of MNNG on ATP and AMP levels were blocked by co-treatment with PJ34 or DPQ, and also blocked by post-treatment with NAD+ or pyruvate.